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Updated: Aug 2, 2026

Examination of the Telomere G-overhang Structure in Trypanosoma brucei
Published on: January 27, 2011
Compositional compartmentalization of the nuclear genomes of Trypanosoma brucei and Trypanosoma equiperdum
A Isacchi1, G Bernardi, G Bernardi
1Laboratoire de Génétique Moléculaire, Institut Jacques Monod, Paris, France.
Abstract:
High molecular weight DNA preparations from Trypanosoma brucei and Trypanosoma equiperdum were fractionated by preparative centrifugation in a Cs2SO4 density gradient in the presence of BAMD, bis(acetatomercurimethyl)dioxane, a sequence-specific DNA ligand. Analytical centrifugation in CsCl of the DNA fractions so obtained showed that both DNAs had a bimodal distribution with two major peaks banding at 1.702-1.703 and 1.708 g/cm3 and representing 1/3 and 2/3 of total DNA, respectively. Several minor components were also detected. These results indicate that a compositional compartmentalization is not only found in the genome of vertebrates and plants, as already described, but also in those of protozoa such as Trypanosomes.
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