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Preparation, characterization and release of microencapsulated bromodeoxyuridine
D Maysinger1, J Filipović-Grcić, T Alebić-Kolbah
1McGill University, Department of Pharmacology and Therapeutics, Montreal, Canada.
Life Sciences
|January 1, 1994
Summary
Biodegradable microspheres loaded with bromodeoxyuridine (BrUrd) were created and tested. These microspheres efficiently deliver BrUrd to proliferating cells near brain lesions in rats.
Area of Science:
- Biomaterials Science
- Drug Delivery Systems
- Neuroscience
Background:
- Microsphere technology offers potential for targeted drug delivery.
- Bromodeoxyuridine (BrUrd) is a thymidine analog used to label proliferating cells.
Purpose of the Study:
- To prepare and characterize biodegradable microspheres containing BrUrd.
- To evaluate the in vitro and in vivo performance of these BrUrd-loaded microspheres for cell proliferation studies.
Main Methods:
- Scanning electron microscopy (SEM) and image analysis for microsphere morphology and size.
- High-performance liquid chromatography (HPLC) for determining BrUrd content.
- In vitro release kinetics studies (first-order and biphasic first-order kinetics).
- In vivo immunocytochemical studies in a rat brain lesion model.
Main Results:
- Microspheres exhibited regular spherical shapes with an average size of 2.47 +/- 0.59 microns.
- BrUrd content ranged from 0.2-1.5% with consistent release kinetics (approx. 12 hr half-time).
- Efficient incorporation of BrUrd into nuclei of proliferating cells near brain lesions was observed.
Conclusions:
- Biodegradable and biocompatible microspheres for BrUrd delivery have been successfully developed.
- These microspheres demonstrate controlled release and effective in vivo delivery for labeling proliferating cells in brain lesions.