[The isolation of Vibrio alginolyticus bacteriophage]

We identified 4 bacteriophages of V. alginolyticus in 29 ones, which were first isolated from seafood. According to their character of the plaques, they were classified into two kinds: one plaque was clear, the other was opaque. The size of these plaques were different and their diameters are 0.5-3.0mm. By electron microscopy observation, they could be classified into two kinds; one has a long axie and hexagemal head, and a thin-long tail, the other has an equal axie hexagenal head and a very short tail, but the edges and corners aren't clear. The multiplication valence of the phages attained to 10(8-9) pfu/ml. Total lysis rate of 4 bacteriophages was 72.22% to V. alginolyticus. However, the lysis rate of single phage was 9.72-44.4%. 4 bacteriophages all had high host specificity. Cross-lysis reaction wasn't found in the test of original solution of bacteriophages to 612 strains of different genus bacteria and 697 strains of genus Vibrio, but they only showed 39% cross-lysis rate to V. parahaemolyticus and most part of this phenomenon disappeared at 10 RTD of the phages. Thus, the obvious relation of consanguinity was showed between two kinds of bacteria strains.