Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tissue printing and its applications in self-incompatibility studies

M Cappadocia1, P Heizmann, C Dumas

  • 1Institut de Recherche en Biologie Végétale, Université de Montréal, Québec, Canada.

Plant Molecular Biology
|December 1, 1993
PubMed
Summary

This study adapted tissue printing to detect self-incompatibility S-locus gene products in female tissues of Brassica and Solanum plants. The technique successfully differentiated S-allele types and quantified S-RNase message levels.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Syngas biomethanation: Study of process performances at high syngas flow rate in pressurized stirred column.

Bioresource technology·2023
Same author

Syngas biomethanation: In a transfer limited process, is CO inhibition an issue?

Waste management (New York, N.Y.)·2023
Same author

"Biomethanation of syngas by enriched mixed anaerobic consortium in pressurized agitated column".

Bioresource technology·2021
Same author

Dynamic observation of the biodegradation of lignocellulosic tissue under solid-state anaerobic conditions.

Bioresource technology·2015
Same author

Substrate milling pretreatment as a key parameter for Solid-State Anaerobic Digestion optimization.

Bioresource technology·2014
Same author

Figurative and spatial information and search behavior in dogs (Canis familiaris).

Behavioural processes·2014

Area of Science:

  • Plant reproductive biology
  • Molecular genetics
  • Biotechnology

Background:

  • Self-incompatibility (SI) is a genetic mechanism preventing self-fertilization in flowering plants.
  • The S-locus genes control SI, with different mechanisms in sporophytic (e.g., Brassica) and gametophytic (e.g., Solanum) systems.
  • Detecting S-locus gene products is crucial for understanding plant reproduction and breeding.

Purpose of the Study:

  • To adapt and apply the tissue printing technique for rapid localization of S-locus gene products in female reproductive tissues.
  • To compare the utility of tissue printing in sporophytic and gametophytic SI systems.
  • To analyze S-RNase message dynamics in Solanum flowers.

Main Methods:

  • Tissue printing technique adapted from Brassica oleracea (sporophytic SI) to Solanum chacoense (gametophytic SI).

Related Experiment Videos

  • Localization of specific mRNA representing S-locus gene products in female tissues.
  • Scanning laser densitometry used to quantify S-RNase message levels in Solanum.

    • Main Results:

    • Tissue printing successfully localized S-locus gene products in both Brassica and Solanum.
    • In Brassica, the method differentiated between class 1 (dominant) and class 2 (recessive) S-alleles.
    • In Solanum, S14 S-RNase message levels declined faster than S13 message levels in mature flowers.

    Conclusions:

    • Tissue printing is a rapid and effective method for detecting S-locus gene products in diverse plant SI systems.
    • The technique provides insights into the molecular mechanisms of both sporophytic and gametophytic self-incompatibility.
    • This method has potential applications in plant breeding and genetic studies of reproduction.