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Determination of L-methionine-dl-sulphoxide in tissue extracts
P W Scislowski1, I Harris, K Pickard
1Rowett Research Institute, Bucksburn, Aberdeen, Scotland, UK.
Journal of Chromatography
|September 22, 1993
Abstract:
The amino acid fraction from rat liver, heart and skeletal muscle was prepared by the separation of sulphosalicylic acid extract on Dowex 50 H+ form. The presence of L-methionine-dl-sulphoxide in these extracts was identified and compared by three independent chromatographic methods: ion-exchange, Pico-Tag and reversed-phase high-performance liquid chromatography after precolumn derivatisation with diethylethoxymethylenemalonate. Quantitative data indicate that L-methionine-dl-sulphoxide is present in the intracellular pool at the levels of free methionine.