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[Demonstration of an endo-beta-N-acetyl-glucosaminidase in rabbit serum]
Comptes Rendus Hebdomadaires Des Seances De L'Academie Des Sciences. Serie D: Sciences Naturelles
|October 18, 1976
Abstract:
An endo-beta-N-acetyl glucosaminidase of rabbit serum has been characterized after purification by affinity chromatography. The specificity was determined by fluorimetric monitoring of the hydrolysis of 4-methylumbelliferyl-beta-glycosides and by thin layer chromatography identification of the hydrolysis products of fluoresceinyl-beta-glycosides. This enzyme acts on the following oligosaccharides GlcNac-beta-1-4 leads to GlcNAc-beta-O-R giving free di-N-acetyl chitobiose and R-OH as hydrolytic products.