[The effect of 1-isoproterenol on monovalent cation transport in activated human lymphocytes]

Potassium (rubidium) influx, sodium and potassium contents, as well as protein contents and 3H-thymidine incorporation have been investigated in quiescent lymphocytes, PHA-stimulated lymphocytes and T-lymphocytes, activated by human recombinant interleukin 2 (IL-2) within 0.5, 24 and 48 h. In quiescent lymphocytes ouabain-sensitive Rb+ influx was diminished by I-isoproterenol and forskolin. Both the cAMP elevating agents decreased the enhancement of ouabain-sensitive and ouabain-resistant Rb+ influxes in PHA-stimulated lymphocytes in 24 and 48 h. The IL-2 activation of T-cells during 24 and 48 h was accompanied by the elevation of Rb+ influxes and Ki content. L-isoproterenol inhibited ouabain-sensitive Rb+ influx in activated T-cells. This inhibition was found to correlate with 3H-thymidine incorporation in cells. Thus, the effects of l-isoproterenol and forskolin on ionic transport in human activated lymphocytes correlated with a well-known cAMP inhibition of lymphocyte proliferation.