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Updated: Feb 26, 2026

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
Published on: August 12, 2019
Cysteine-Containing Peptides Stimulate Monocyte Migration through NADPH-Oxidase Activation
T L Krasnikova1, T I Arefieva2, E A Pylaeva2
1Russian Cardiology Research and Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia. tlkrasnikova@gmail.com.
Abstract:
We analyzed migration of monocytes under the effect of apocinin (NADPH inhibitor) and PD98059 (blocker of extracellular MEK/ERK kinase involved in Nox4 oxidase-mediated migration of monocytes). Migration of monocytes stimulated by cysteine-containing peptides (fragments of chemokines with free thiol group MCP-1 and fractalkine) was completely inhibited by apocinin and MEK/ERK blocker. It is assumed that the stimulating effect of cysteine-containing peptides on monocyte migration is mediated by the NADPH-oxidase system, in particular, Nox4.
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