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DNA segments mapped by reciprocal use of microsatellite primers between mouse and rat
1Institute of Laboratory Animals, Faculty of Medicine, Kyoto University, Japan.
Abstract:
Rat microsatellite primers were used for detection of homologous DNA segments in the mouse species (Mus laboratorius, Mus musculus musculus, and Mus spretus). Twenty five (16.3%) of 153 rat primer pairs amplified specific DNA segments, when genomic DNA of mice was used as a template in the polymerase chain reaction (PCR). Size variation among inbred strains of mice was found for 13 DNA segments (8.5%). Eight out of the 13 polymorphic DNA segments were mapped to a particular chromosome with two sets of recombinant inbred strains, AKXL or BXD. Similarly, mouse microsatellite primers were used for detection of homologous DNA segments in rats (Rattus norvegicus). Twenty (12.0%) of 166 primer pairs amplified specific DNA segments from rat genome. Size variation among inbred strains of rats was found for seven DNA segments (4.2%). Eleven of these 20 DNA segments were mapped with a rat x mouse somatic cell hybrid clone panel and/or linkage analysis by use of backcross progeny. Our results suggest that the mapped DNA segments are really homologs between mouse and rat. These polymorphic DNA segments are useful genetic markers.
Insights
Researchers identified homologous DNA segments between rats and mice using microsatellite primers. These polymorphic DNA segments serve as valuable genetic markers for comparative genomics studies.
Area of Science:
- Comparative genomics
- Molecular genetics
Background:
- Microsatellite markers are widely used for genetic mapping in various species.
- Understanding conserved DNA segments between rodents aids in comparative genetic studies.
Purpose of the Study:
- To identify and map homologous DNA segments between rat (Rattus norvegicus) and mouse (Mus species) genomes.
- To assess the utility of these homologous segments as genetic markers.
Main Methods:
- Utilized rat microsatellite primers to screen mouse genomic DNA via polymerase chain reaction (PCR).
- Employed mouse microsatellite primers to screen rat genomic DNA.
- Mapped polymorphic DNA segments using recombinant inbred strains and somatic cell hybrid panels.
Main Results:
- 16.3% of rat primer pairs amplified specific mouse DNA segments, with 8.5% showing polymorphism.
- 12.0% of mouse primer pairs amplified specific rat DNA segments, with 4.2% showing polymorphism.
- Successfully mapped several homologous DNA segments to specific chromosomes in both species.
Conclusions:
- Rat and mouse genomes share homologous DNA segments detectable by microsatellite markers.
- These identified polymorphic homologous DNA segments are valuable genetic markers for cross-species comparative mapping and genetic research.