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Related Experiment Videos

Antifungal susceptibility testing

J H Rex1, M A Pfaller, M G Rinaldi

  • 1Center for Infectious Diseases, University of Texas Medical School at Houston 77030.

Clinical Microbiology Reviews
|October 1, 1993
PubMed
Summary
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Reliable antifungal susceptibility testing remains challenging due to variable results. A proposed standard method for Candida and Cryptococcus aims to minimize interlaboratory variability in antifungal testing.

Area of Science:

  • Clinical Microbiology
  • Mycology
  • Antimicrobial Resistance

Background:

  • Antifungal susceptibility testing (AFST) is less established than antibacterial testing.
  • Numerous factors (pH, inoculum, medium, incubation) significantly impact AFST results, causing discrepancies.
  • Poor interlaboratory agreement is common in current AFST methods.

Purpose of the Study:

  • To review the development of a standardized AFST method.
  • To address the challenges in achieving reliable antifungal susceptibility testing.
  • To propose a standard method for testing Candida spp. and Cryptococcus neoformans.

Main Methods:

  • Extensive collaborative projects were conducted to study the effects of various testing parameters.
  • The Subcommittee on Antifungal Susceptibility Testing of the National Committee for Clinical Laboratory Standards played a key role.

Related Experiment Videos

  • A standard method was developed and proposed to minimize interlaboratory variability.

    • Main Results:

    • A proposed standard method has been developed for AFST of Candida and Cryptococcus.
    • This method aims to reduce variability caused by factors like pH, inoculum size, and incubation conditions.
    • The proposed standard shows promise in improving interlaboratory agreement.

    Conclusions:

    • Standardization is crucial for reliable AFST.
    • The proposed method offers a way to improve consistency in testing Candida and Cryptococcus.
    • Further work is needed to refine and validate the proposed AFST standard.