Bacteriology of peritoneal fluid in paediatric surgical emergencies

This study deals with the incidence of aerobic and anaerobic organisms of the peritoneal fluid of 60 consecutive children subjected to emergency celiotomy. Two-thirds of the cases were below the age of year. Peritoneal fluid was collected immediately after entering the peritoneal cavity and inoculated both for aerobic an anaerobic cultures. Bacterial growth occurred in 34 (57%) of the specimens. Anaerobic bacteria alone grew in 3 (5%) of the specimens, aerobes alone in 22 (37%) of specimens. A mixed aerobic and anaerobic bacteria grew in 9 (15%) of the specimens. There were 44 aerobic and 19 anaerobic isolates. All anaerobes were sensitive to metronidazole while most of the aerobes were sensitive to gentamycin. Culture was positive in 20 out of 42 (47.6%) specimens, where the peritoneal fluid was clear and there was no evidence of peritoneal contamination at the time of surgery.