Related Experiment Video
Updated: Aug 16, 2026

A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies
Published on: November 20, 2014
The value of quantitative protein electrophoresis
Abstract:
Study of serum protein fractions separated by electrophoresis shows variation in subjects of different sex and race. Interpretation of results by clinicians is often difficult since the bands are composed of several different proteins, each responding to different physiological stimuli. Important information can be gained by examining the electrophoretogram and the qualitative appearance of the bands. This should be communicated to the clinician and, depending on the clinical and qualitative electrophoretic findings, an analysis of certain specific proteins should be performed.
More Related Videos
07:58Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
Published on: September 19, 2018
08:13Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
Published on: April 9, 2019
Related Concept Videos
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Electrophoresis: Overview
There...