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Low-temperature scanning electron microscopy in fungus-nematode interaction
E den Belder1, A Boekestein, J W van Esch
1Department of Biological and Integrated Control, DLO Research Institute for Plant Protection (IPO-DLO), Wageningen, The Netherlands.
Scanning
|January 1, 1993
Abstract:
A comparative study has been made of different preparation techniques used for the scanning electron microscope (SEM), with regard to their application to fungus-nematode interaction. The preparation of frozen-hydrated specimens of both healthy and Arthrobotrys-oligospora-infected second-stage larvae of the root-knot nematode (Meloidogyne sp.) is described, and the results are compared with those obtained by critical point-drying and freeze-drying. In all cases the frozen-hydrated specimens consistently showed the best preservation.