Related Experiment Videos
Induction and distribution of cellular retinol-binding protein, type two during villus-crypt development in the chick
1Department of Nutrition, School of Food and Nutritional Sciences, University of Shizuoka, Japan.
Insights
Cellular retinol-binding protein, type two (CRBP(II)) levels significantly increase in chick duodenum during the perinatal period and after hatching. These changes in CRBP(II) likely relate to dietary vitamin A absorption.
Area of Science:
- Animal science
- Developmental biology
- Nutritional science
Background:
- Cellular retinol-binding protein, type two (CRBP(II)) plays a vital role in vitamin A metabolism.
- Understanding CRBP(II) expression is crucial for avian development and nutrition.
Purpose of the Study:
- To quantify the developmental changes in CRBP(II) levels in the chick duodenum.
- To investigate the relationship between CRBP(II) distribution and the onset of food intake.
Main Methods:
- Quantitative analysis of CRBP(II) protein levels.
- Examination of duodenal villi during embryonic and posthatch development.
Main Results:
- A >10-fold increase in CRBP(II) was observed in lower duodenal villi between day 18 of embryogenesis and 1 day posthatch.
- A subsequent >2-fold increase in CRBP(II) occurred in mid-villus enterocytes with postnatal growth and feeding.
Conclusions:
- CRBP(II) is first induced in differentiating lower duodenal enterocytes during the perinatal period.
- Further increases in CRBP(II) in mid-villus cells coincide with the appearance of retinol in the duodenal lumen, suggesting a role in dietary vitamin A absorption.
Abstract:
Between day 18 of embryogenesis and 1 day posthatch, a rapid and remarkable (over 10-fold) increase in cellular retinol-binding protein, type two (CRBP(II)) levels occurred in the lower portion of duodenal villi of the chick. With further postnatal growth and concomitant with initiation of food intake, CRBP(II) levels increased over 2-fold in the mid-villus enterocytes. The results suggest that a first induction of CRBP(II) occurs in differentiating lower villus enterocytes during the perinatal period and is followed by marked increases in mid-villus cells. These quantitative change in the distribution and levels of CRBP(II) are probably related to the appearance of retinol in the duodenal lumen.