The self-association of ovine erythrocyte spectrin

1. Spectrin extracted from ovine erythrocyte membranes at low temperature shows association behaviour similar to that reported for human and bovine erythrocytes. 2. The spectrin tetramer is the predominant oligomer, the dimer is well represented, and smaller amounts of hexamer and higher oligomers are present. 3. The estimates of parameters describing the self-association of purified ovine spectrin studied by sedimentation equilibrium analysis were found to be indistinguishable from those obtained for human spectrin under the same conditions, within the precision of the measurements. 4. The data suggest that the cooperative isodesmic model may be general for spectrin, and not a peculiarity of the human.