[Fast identification of Salmonella by chemiluminescent photographic detection]

Salmonella anatis was identified by the chemiluminescent photographic detection technique established in this lab. The horseradish peroxidase labelled antibody was incubated with the bacteria immobilized on nitrocellulose film; after washing, the film was contacted with a filter paper with absorbed chemiluminescence substrate solution. Twenty minutes of exposure of the chemiluminescence produced to X-ray film gave the positive results. As few as one hundred bacteria could be detected in about 2h. The tests showed that the specificity of the method is good.