[Design of recombinant DNA intended for insertional mutagenesis of bacteria]

The recombinant plasmid pKC47M has been constructed on the base of a mini-Mu-phage and the replicon of plasmid pBR328. The plasmid was constructed for in vivo insertional mutagenesis in bacteria. Use of the plasmid in vivo alleviates the possibility of secondary transposition of the inserted sequences and enrichment of multiple-site mutations. Efficient mutagenesis caused by plasmid pKC47M was tested by induction auxotrophic mutations in Escherichia coli, Yersinia enterocolitica, Yersinia pseudotuberculosis.