[Selective photoimmunoinactivation of influenza virus]

Antibodies to influenza virus were conjugated with hematoporphyrin. The conjugates were verified by the peak point identity in protein absorption (lambda-280 nm) of elution profiles and by the porphyrin-specific fluorescence. The antibody conjugates have been shown to preserve the specific antigenic activity tested by enzyme immunoassay. The conjugated hematoporphyrin nuclei have also preserved the photochemical activity since the antibody conjugates lose their native properties being exposed to visible light. In the neutralization test in the dark the conjugates of specific antibodies with hematoporphyrin decrease the viral cultures infectivity to the same extent as nonconjugated antibodies. This fact verifies antibodies stability in the conjugates. The infectivity of viral cultures exposed to visible light after neutralization test was further decreased by several orders. The data suggest undoubtedly perspective use of this method in viral infections control.