Related Experiment Video
Updated: Aug 15, 2026

Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates
Published on: February 24, 2011
PFGE MAPPER: a tool to aid in the analysis of pulse field gel electrophoresis maps
1Center for Medical Informatics, Yale University School of Medicine, New Haven, CT 06510.
Abstract:
Pulse field gel electrophoresis mapping is an important technique for characterizing large segments of DNA and constructing long-range restriction maps. We have developed a tool, PFGE MAPPER, to aid in the construction of pulse field electrophoresis gel maps. This tool helps construct pulse field gel maps from single and double digest experiments visualized by hybridization with single copy probes. The program is written in Think C and runs on Macintosh computers. An intuitive interface allows the user to interactively modify fragment sizes or errors, select fragments for analysis and recalculate the maps. Maps can be printed or saved for later viewing. After constructing and saving several maps in a region, PFGE MAPPER can be used to refine and extend the overall map by merging individual maps. This tool should be useful for constructing long-range restriction maps of genomic DNA and yeast artificial chromosomes.
Related Concept Videos
DNA Agarose Gel Electrophoresis
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Electrophoresis: Overview
There...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

