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Updated: Sep 14, 2026

Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction
Published on: May 7, 2015
[Anoxia-reperfusion injury to endothelial cells: mechanism and protection]
S Yu1
1Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xian.
Abstract:
Endothelial cells in the aortic walls of inbred SD rats were cultured in MEM culture medium containing 10% calf serum, and an anoxia-reperfusion injury model was established. The effect of anoxia-reperfusion injury on cultured endothelial cells was studied by measurements of membrane fluidity, intracellular Ca content, the release rate of 51Cr and the uptake rate of trypan-blue. The effect of fructose 1-6 diphosphate (FDP) and captopril (Cap) on cultured endothelial cells was also investigated. The findings indicated that the two drugs might protect cultured endothelial cells from anoxia-reperfusion injury. The mechanism of anoxia-reperfusion injury and the protective effect of the two drugs on cultured endothelial cells were briefly discussed.
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