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Patterned neuronal attachment and outgrowth on surface modified, electrically charged fluoropolymer substrates
R F Valentini1, T G Vargo, J A Gardella
1Section of Artificial Organs, Biomaterials and Cellular Technology, Brown University, Providence, RI 02912.
Journal of Biomaterials Science. Polymer Edition
|January 1, 1993
Summary
Bulk electrical charges on fluorinated ethylenepropylene copolymer (FEP) and polyvinylidene fluoride (PVDF) significantly enhance neuronal differentiation. Surface modifications modulate cell morphology and attachment, suggesting combined properties are key for neural regeneration templates.
Area of Science:
- Biomaterials Science
- Neuroscience
- Surface Chemistry
Background:
- Fluorinated ethylenepropylene copolymer (FEP) and polyvinylidene fluoride (PVDF) can acquire electrical charges through bulk molecular rearrangements.
- Electrically active FEP and PVDF substrates have previously shown to promote nerve fiber outgrowth in cultured neurons.
Purpose of the Study:
- To investigate if surface modifications with charged groups (hydroxyl and amine) alter the effect of bulk electrical charges on neuronal cells.
- To determine the role of surface chemistry in modulating neuronal responses to electrically active polymers.
Main Methods:
- Mouse neuroblastoma (Nb2a) cells were cultured on FEP and PVDF substrates with charged (OH, NH2) and uncharged surfaces.
- Surface modifications were applied uniformly or in striped patterns.
- Neuronal differentiation, morphology, and attachment were analyzed.
Main Results:
- Nb2a cells exhibited enhanced differentiation on electrically active FEP and PVDF compared to neutral substrates.
- Amine (NH2) groups attenuated the differentiation response in serum-containing media.
- Cells showed preferential attachment to NH2-modified regions, particularly in striped patterns, and displayed flatter morphology.
Conclusions:
- Bulk electrical charges play a more significant role than surface charges in stimulating neuronal differentiation.
- Surface functional groups modulate neuronal morphology and attachment properties.
- Optimal neuronal regeneration templates likely require a combination of specific bulk and surface properties.