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Related Experiment Videos

Basic fibroblast growth factor modulates integrin expression in microvascular endothelial cells

S Klein1, F G Giancotti, M Presta

  • 1Department of cell Biology, New York University Medical Center, New York.

Molecular Biology of the Cell
|October 1, 1993
PubMed
Summary
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Basic fibroblast growth factor (bFGF) significantly alters integrin expression and function in microvascular endothelial cells. This promotes enhanced cell adhesion to extracellular matrix components, crucial for angiogenesis.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Angiogenesis Research

Background:

  • Endothelial cells modify extracellular matrix interactions during angiogenesis.
  • Integrins are key cell surface receptors mediating cell-matrix adhesion.
  • Basic fibroblast growth factor (bFGF) is a potent angiogenic factor.

Purpose of the Study:

  • To investigate the effect of bFGF on integrin expression and function in microvascular endothelial cells.
  • To understand how bFGF modulates integrin profiles during angiogenesis.

Main Methods:

  • Immunoprecipitation assays to identify expressed integrins.
  • Treatment of endothelial cells with bFGF.
  • Analysis of integrin subunit biosynthesis and surface expression.

Related Experiment Videos

  • Cell adhesion assays to assess functional changes.

    • Main Results:

    • bFGF significantly increased surface expression of several integrins (e.g., α2β1, α3β1, α5β1, α6β1, α6β4, αvβ5).
    • bFGF decreased expression of α1β1 and αvβ3 integrins.
    • bFGF altered the biosynthesis of specific integrin subunits (α3, α5, α6, β4, β5, αv, β3).
    • bFGF-treated cells exhibited enhanced adhesion to fibronectin, laminin, vitronectin, and collagen.
    • Upregulation of β1 integrins and endoCAM occurred around 72 hours post-bFGF exposure.

    Conclusions:

    • bFGF modulates integrin expression in microvascular endothelial cells by altering subunit biosynthesis.
    • These changes in integrin expression lead to improved endothelial cell adhesion.
    • bFGF-induced integrin modulation is a significant component of the angiogenic process.