Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Intracellular pH changes during zona drilling

H T Depypere¹, L Leybaert

¹University Women's Clinic, University Gent, Belgium.

Fertility and Sterility

|February 1, 1994

Summary

Zona drilling significantly lowers intracellular pH in both mouse and human oocytes, causing temporary cytoplasmic acidification. This pH change may contribute to oocyte degeneration observed after the procedure.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A new look at osteoarthritis: Threshold potentials and an analogy to hypocalcemia.

Frontiers in aging·2023

Same author

Assisted oocyte activation does not overcome recurrent embryo developmental problems.

Human reproduction (Oxford, England)·2023

Same author

Comparative study of preimplantation development following distinct assisted oocyte activation protocols in a PLC-zeta knockout mouse model.

Molecular human reproduction·2020

Same author

Human oocyte calcium analysis predicts the response to assisted oocyte activation in patients experiencing fertilization failure after ICSI.

Human reproduction (Oxford, England)·2018

Same author

Fluoxetine suppresses calcium signaling in human T lymphocytes through depletion of intracellular calcium stores.

Cell calcium·2015

Same author

The serum estradiol concentration is the main determinant of the estradiol concentration in normal breast tissue.

Maturitas·2015

Area of Science:

Reproductive biology
Cellular physiology

Background:

Zona drilling is an assisted reproductive technology.
Intracellular pH plays a critical role in oocyte function and development.

Purpose of the Study:

To determine if zona drilling impacts intracellular pH in oocytes.
To investigate potential mechanisms for oocyte degeneration post-zona drilling.

Main Methods:

Intracellular pH measurement in mouse oocytes using pH-sensitive microelectrodes.
Intracellular pH measurement in human oocytes using microfluorimetry with a pH-sensitive dye.

Main Results:

Mouse oocytes showed a drop in intracellular pH from 7.25 to 7.09 during zona drilling, with recovery within 4 minutes.
Human oocytes experienced an average intracellular pH drop of 0.36 units, also recovering within 4 minutes.
Significant cytoplasmic acidification was observed in both species.

Conclusions:

Zona drilling induces significant cytoplasmic acidification in both mouse and human oocytes.
This acidification may be linked to the increased rate of cytoplasmic degeneration observed in human oocytes after zona drilling.

Related Experiment Videos