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Generation of Murine Monoclonal Antibodies by Hybridoma Technology
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An immuno-dominant sequential epitope recognized by mouse anti-recombinant bovine growth hormone antisera

J Beattie1, V Borromeo, H A Fawcett

  • 1Hannah Research Institute, Ayr, Scotland, UK.

Immunology Letters
|September 1, 1993
PubMed

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Overview
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Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...