[Restriction analysis of chromosomal DNA from strains of Salmonella enteritidis]

Restriction analysis of the chromosomal DNA from 17 Salmonella enteritidis strains isolated in Moscow and Novomoskovsk (Tula region) during epidemics and sporadically has been undertaken. Restriction profiles of the studied strains divide them into five groups differing in physical structure of chromosomal DNA which is expressed in the presence of supplementary and more intensively stained DNA fragments, possibly, amplified. Restriction analysis is hindered by the presence of plasmids in the strains and demands a parallel analysis of the plasmid DNA carried by the strain. Lack of coincidence of strain groups obtained by different methods demands further investigation to define the real significance of the different methods for Salmonella enteritidis typing.