Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An improved MTT assay using the electron-coupling agent menadione

H Garn1, H Krause, V Enzmann

  • 1Department of Biosciences, University of Leipzig, Germany.

Journal of Immunological Methods
|February 10, 1994
PubMed
Summary

This study enhances the MTT assay for improved cytotoxicity testing. Adding menadione boosts formazan formation in splenocytes and tumor cells, aiding macrophage-based assays.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Implementation of exclusive enteral nutrition in pediatric Crohn's disease: a secondary analysis of the CLARA registry study.

Frontiers in pediatrics·2026
Same author

[Implementation and benefits of healthcare apps in surgical disciplines-A Delphi expert consensus].

Chirurgie (Heidelberg, Germany)·2025
Same author

[Trauma-associated vascular injuries and the vascular surgical/interventional options for vascular reconstruction].

Chirurgie (Heidelberg, Germany)·2024
Same author

New Constraint on the Local Relic Neutrino Background Overdensity with the First KATRIN Data Runs.

Physical review letters·2022
Same author

[Complication rate after femoral shaft fractures in childhood and adolescence depending on patient factors and treatment measures].

Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2021
Same author

Bound on 3+1 Active-Sterile Neutrino Mixing from the First Four-Week Science Run of KATRIN.

Physical review letters·2021

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • The MTT assay is a common method for assessing cell viability and proliferation.
  • Tetrazolium-based assays are widely used in immunology and cancer research.
  • Enhancing the sensitivity of these assays is crucial for accurate cytotoxicity evaluation.

Purpose of the Study:

  • To describe a modification of the MTT assay using an electron-coupling agent.
  • To investigate the effect of menadione on formazan formation in different murine immune and tumor cells.
  • To assess the potential improvement of tetrazolium-based cytotoxicity assays for murine macrophages.

Main Methods:

  • Modification of the MTT (tetrazolium) colorimetric assay.
  • Utilizing the electron-coupling agent menadione.

Related Experiment Videos

  • Assessing formazan formation in murine splenocytes, P-815 cells, and macrophages.

    • Main Results:

    • Menadione significantly increased formazan formation in murine splenocytes and P-815 cells.
    • Dye reduction by macrophages was minimally affected by menadione.
    • The modification shows potential for enhancing tetrazolium-based cytotoxicity assays.

    Conclusions:

    • The addition of menadione can enhance formazan production in specific cell types within the MTT assay.
    • This modification may improve the sensitivity of macrophage-mediated cytotoxicity assays against tumor cells.
    • Further optimization could lead to more robust assessments of immune cell function and anti-cancer activity.