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A mouse model for studying epidermodysplasia-verruciformis-associated carcinogenesis
S Majewski1, F Breitburd, M Skopinska
1Department of Dermatology, Warsaw School of Medicine, Poland.
International Journal of Cancer
|March 1, 1994
Summary
Epidermodysplasia verruciformis (EV) is a genetic cancer model. A new in vivo system successfully propagated EV tissue, enabling study of human papillomavirus (HPV) in skin cancer progression.
Area of Science:
- Dermatology
- Oncology
- Virology
Background:
- Epidermodysplasia verruciformis (EV) presents unique susceptibility to human papillomaviruses (HPVs), serving as a genetic cancer model.
- Understanding EV pathogenesis and HPV's role in cutaneous carcinogenesis is crucial.
Purpose of the Study:
- To establish a novel in vivo experimental system for long-term propagation of EV tissue.
- To facilitate the study of tumor progression in Epidermodysplasia verruciformis.
Main Methods:
- Grafting skin fragments from EV patients' lesions onto athymic mice.
- Long-term observation of grafted tissue under the kidney capsule.
- Analysis of grafted tissue using in situ hybridization and immunoperoxidase staining.
Main Results:
- Successful formation of epidermal cysts from grafted EV tissue.
- Detection of specific HPV types (5, 8, 9, 12, 36) and papillomavirus antigens within the cysts.
- Observation of proliferative changes in some cysts, indicative of early carcinogenesis.
Conclusions:
- The developed in vivo system effectively models EV tissue propagation.
- This system is valuable for investigating HPV's role and co-factors in human skin cancer development.