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Related Experiment Videos

Antigen-driven B cell differentiation in vivo

M G McHeyzer-Williams1, M J McLean, P A Lalor

  • 1Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.

The Journal of Experimental Medicine
|July 1, 1993
PubMed
Summary
This summary is machine-generated.

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This study reveals that antibody-secreting cells and memory B cells originate from distinct pathways after T cell-dependent antigen challenge. Somatic hypermutation primarily occurs in germinal center B cells, not antibody-secreting cells.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • T cell-dependent antigen challenge induces antibody secretion and memory B cell development.
  • Germinal centers are crucial sites for B cell maturation, somatic hypermutation, and memory formation.
  • Understanding the distinct pathways of B cell differentiation is key to immune response efficacy.

Purpose of the Study:

  • To quantitatively assess B cell recruitment into antibody-secreting and memory cell compartments.
  • To investigate the timing and location of somatic hypermutation in B cells.
  • To explore potential selection events influencing B cell fate.

Main Methods:

  • Six-parameter flow cytometry for B cell analysis.
  • Single-cell molecular analysis to track somatic hypermutation.

Related Experiment Videos

  • Quantitative assessment of cellular expansion and differentiation.

    • Main Results:

    • Cellular expansion in antibody-secreting and germinal center pathways is initially exponential and independent.
    • Somatic hypermutation begins late in the first week and is confined to the germinal center pathway.
    • Germinal center B cells exhibit shorter CDR3 regions, suggesting selection, even before mutation.

    Conclusions:

    • Significant B cell proliferation occurs in germinal centers before somatic hypermutation initiation.
    • A distinct selection event may occur at the pathway bifurcation, favoring cells with shorter CDR3.
    • Mutated B cell clonotypes with shorter CDR3 and affinity-increasing mutations are selected into the memory compartment.