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Related Experiment Videos

Undifferentiated carcinoma: an immunohistochemical and ultrastructural study

Z Suo1, H Qvist, W Su

  • 1Department of Pathology, Norwegian Radium Hospital, Oslo.

Anticancer Research
|May 1, 1993
PubMed
Summary

This study investigated undifferentiated carcinomas (UCs) using immunohistochemistry. Key findings include frequent expression of cytokeratins (CKs) 8, 18, and 19, with vimentin and p53 protein also noted in a significant portion of UCs.

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Area of Science:

  • Oncology
  • Pathology
  • Molecular Biology

Background:

  • Undifferentiated carcinomas (UCs) present diagnostic challenges.
  • Immunohistochemistry aids in characterizing tumor cell differentiation.

Purpose of the Study:

  • To investigate the expression profile of cytokeratins (CKs), vimentin, and p53 protein in undifferentiated carcinomas (UCs).
  • To evaluate the utility of these markers in the diagnosis and differentiation of UCs.

Main Methods:

  • Immunohistochemical analysis of 28 UCs using antibodies against CKs (CK8, 18, 19, 5/6, 20, AE1/AE3), vimentin, p53 protein, c-erbB-2 protein, and CEA.
  • Conventional histopathology and electron microscopy were used for initial diagnoses.

Main Results:

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  • High frequency of CK8 (61%), CK18 (61%), and CK19 (82%) expression in UCs.
  • CK5/6 was positive in 32% and CK20 in 11% of cases.
  • P53 protein overexpression was observed in 32% of UCs; vimentin was expressed in 32%, including all thyroid UCs.
  • Vimentin and CK18 were more frequently expressed in UCs compared to squamous cell carcinomas.
  • P53 protein overexpression showed similar rates in UCs and squamous cell carcinomas, suggesting it's not a differentiation marker.

    • Conclusions:

    • A combination of cytokeratin antibodies (CK cocktail) is recommended for diagnosing UCs.
    • Vimentin and specific CK profiles can aid in distinguishing UCs.
    • P53 protein overexpression is not a reliable marker for differentiation in these tumor types.