Cell-cycle disruptions and apoptosis induced by the cyclophosphamide derivative mafosfamide

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Published on: June 6, 2017

A N Davidoff1, B V Mendelow

  • 1Department of Haematology, School of Pathology of the University of the Witwatersrand, South Africa.

Experimental Hematology
|July 1, 1993
PubMed

Insights

Mafosfamide, a cyclophosphamide derivative, induces apoptosis and cell cycle arrest in leukemic cells. This suggests potential synergistic effects when combined with other anticancer agents.

Area of Science:

  • Pharmacology
  • Cell Biology
  • Oncology

Background:

  • Cyclophosphamide derivatives are crucial in cancer chemotherapy.
  • Understanding the mechanisms of action of novel agents like mafosfamide is essential.

Purpose of the Study:

  • To investigate the in vitro effects of mafosfamide (ASTA Z 7557) on HL60 leukemic cells.
  • To determine the impact of mafosfamide on cell cycle progression and apoptosis.

Main Methods:

  • Exposure of HL60 cells to varying doses of mafosfamide (0.1-10 µg/mL).
  • Cell cycle analysis using methods like double thymidine block.
  • Morphological and biochemical assessment of apoptosis.

Main Results:

  • Mafosfamide induced S-phase accumulation and suggested G2-phase arrest in unsynchronized cells.
  • Apoptosis was observed in mafosfamide-treated cells, occurring alongside S-phase and G2-phase cell cycle arrests.
  • Significant delay in S-phase progression was noted in synchronized cells.

Conclusions:

  • Mafosfamide triggers apoptosis and cell cycle arrest in leukemic cells.
  • Potential for synergistic activity of mafosfamide with other anticancer drugs that induce similar cell cycle arrests or apoptosis.

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