[Properties of glutamine synthetase of the brain of rats during ontogenesis]

When investigating activity of glutamine synthetase of the enzymatic preparations isolated from the brain of rats of 0.5, 1, 3, 12 and 24-month age, no considerable differences were found in the indices of the values Km to a-glutamate and Vmax which are respectively equal to: Km (M-10(-3))=5.5; 3.5; 3.6; 3.9; 5.5; Vmax=3.1; 4.5; 5.0; 5.2 muM were found. When adding various concentrations of a-ketoglutaric acid into the incubation medium the differences are registered in the degree and character of the age changes in brain glutamine synthetase activity in comparison with this enzyme form the liver.