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Enhanced renal IGF-I expression following partial kidney infarction
S A Rogers1, S B Miller, M R Hammerman
1Department of Internal Medicine, George M. O'Brien Kidney and Urological Diseases Center, Washington University School of Medicine, St. Louis, Missouri 63110.
The American Journal of Physiology
|June 1, 1993
Summary
Partial kidney infarction significantly increases renal insulin-like growth factor (IGF)-I expression in remaining tissue. This enhanced IGF-I synthesis, peaking at 3 days, is crucial for interpreting chronic renal failure models.
Area of Science:
- Nephrology
- Endocrinology
- Molecular Biology
Background:
- Renal insulin-like growth factor (IGF)-I expression is known to increase following kidney mass reduction.
- Understanding the dynamics of IGF-I expression in remnant kidney models is crucial for research on chronic renal failure.
Purpose of the Study:
- To characterize renal IGF-I expression in remnant kidney tissue after partial unilateral infarction.
- To investigate the impact of different extents of kidney mass reduction on IGF-I levels.
Main Methods:
- Partial unilateral kidney infarction was performed in rats, with or without contralateral nephrectomy (1/2NX or 1 1/2NX).
- Levels of extracted IGF-I and IGF-I mRNA were measured in remnant renal tissue at various time points post-infarction.
Main Results:
- IGF-I levels and IGF-I mRNA in remnant renal tissue significantly increased, peaking on day 3 after 1/2NX, and then returned toward baseline.
- A similar pattern of elevated IGF-I and mRNA was observed after 1 1/2NX.
- IGF-I levels in remnant tissue did not correlate with the extent of renal mass reduction; they were similar after 1/2NX and 1 1/2NX.
Conclusions:
- Partial renal infarction acts as a stimulus for enhanced renal IGF-I expression.
- The increase in renal IGF-I appears to result from enhanced synthesis.
- Elevated renal IGF-I must be considered when interpreting studies using remnant kidney models of chronic renal failure.