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Evaluation of current methods for creatine kinase isoenzyme fractionation
Clinical Chemistry
|February 1, 1977
Summary
Electrophoresis offers superior creatine kinase isoenzyme resolution and reliability for diagnostics. Ion-exchange methods provide comparable results with enhanced sensitivity, especially in batch operations, requiring less technical skill.
Area of Science:
- Biochemistry
- Clinical Diagnostics
- Biotechnology
Background:
- Creatine kinase (CK) isoenzymes are crucial biomarkers in diagnosing various medical conditions, including myocardial infarction and muscular dystrophies.
- Accurate fractionation of CK isoenzymes is essential for reliable diagnostic interpretation.
- Several laboratory methods exist for CK isoenzyme analysis, each with distinct advantages and limitations.
Purpose of the Study:
- To evaluate and compare the diagnostic utility of different methods for fractionating creatine kinase isoenzymes.
- To determine the most suitable methods for clinical diagnostic purposes based on resolution, reliability, sensitivity, and technical requirements.
Main Methods:
- Comparative evaluation of electrophoretic, ion-exchange, kinetic, selective activation, and immunological fractionation techniques.
- Assessment of resolution, reliability, and sensitivity using conventional and modified techniques (e.g., film elution).
- Analysis of technical skill requirements and operational time for each method.
Main Results:
- Electrophoretic methods demonstrated superior resolution and reliability but were less sensitive with conventional film-drying.
- Sensitivity of electrophoresis improved to match ion-exchange methods when using film elution.
- Ion-exchange methods offered acceptable resolution and reliability with potentially higher sensitivity and required less technical expertise than electrophoresis.
- Batch ion-exchange operation proved more efficient and sensitive than column operation.
Conclusions:
- Electrophoresis is a highly reliable method for CK isoenzyme fractionation, particularly when sensitivity is enhanced through techniques like film elution.
- Ion-exchange chromatography presents a viable alternative, offering good performance, potentially superior sensitivity, and lower technical demands, especially with batch processing.
- Kinetic, selective activation, and immunological methods were found unsuitable for diagnostic CK isoenzyme fractionation in this evaluation.