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[Disorder of the serum albumin transport function in chronic oxygen starvation and its pharmacological correction]
Insights
Hypoxia in pregnant rats and newborns impaired albumin function and increased bilirubin. Supplements like alpha-tocopherol acetate normalized these effects, improving bilirubin binding to albumin.
Area of Science:
- Biochemistry
- Physiology
- Toxicology
Background:
- Chronic intermittent hypoxia can disrupt normal physiological processes.
- Hypoxia during critical developmental periods may have lasting biochemical consequences.
Purpose of the Study:
- To investigate the biochemical effects of chronic intermittent hypoxia on serum albumin and bilirubin transport in pregnant rats and their offspring.
- To evaluate the efficacy of specific antioxidant and metabolic agents in mitigating hypoxia-induced biochemical alterations.
Main Methods:
- Pregnant rats and their neonates were exposed to chronic intermittent hypoxia.
- Biochemical parameters including serum albumin activity, bilirubin levels, fatty acid binding, and acidosis were assessed.
- In vitro and in vivo studies were conducted to evaluate bilirubin binding to albumin.
- Animals received administration of alpha-tocopherol acetate, nicotinamide, sodium oxybutyrate, and trisamine.
Main Results:
- Hypoxia decreased serum albumin functional activity related to bilirubin transport.
- Accumulation of albumin-bound fatty acids, acetaldehyde, and uncompensated acidosis were observed.
- Brain bilirubin levels increased, and heme oxygenase activity was enhanced in hypoxic rats.
- Supplement administration normalized most biochemical parameters, improving endogenous bilirubin binding to albumin.
Conclusions:
- Chronic intermittent hypoxia significantly disrupts albumin's role in bilirubin transport and causes metabolic disturbances.
- Therapeutic interventions show promise in restoring biochemical homeostasis and improving bilirubin handling under hypoxic conditions.
- Further research into heme oxygenase activity modulation may be warranted.
Abstract:
Grade II chronic intermittent hypoxic hypoxia under normal barometric pressure, which was reproduced in pregnant rats 2.0-2.5 weeks before expected delivery and in the offsprings in the first 2 days of neonatality caused a decrease in serum albumin functional activity associated with bilirubin transport, as evidenced in vitro, as well as accumulation of serum albumin-bound nonesterified higher fatty acids and acetaldehyde along with uncompensated acidosis, an increase in brain levels of bilirubin in adult and newborn rats, and enhancement of its hemoxygenase activity. Administration of alpha-tocopherol acetate, nicotinamide, sodium oxybutyrate, and trisamine to animals exposed to hypoxia normalized the above biochemical parameters, except for hemoxygenase activity and promoted a greater binding of endogenous bilirubin to albumin in adult and newborn rats in vivo.