Enzyme polymorphism of Azorhizobium strains and other stem- and root-nodulating bacteria isolated from Sesbania...

Relationships between bacterial groups nodulating Sesbania rostrata were evaluated through examination of electrophoretic polymorphism of esterases and metabolic enzymes. The following conclusions were drawn: (i) the differentiation of two genomic species within Azorhizobium strains and a group of non-identified strains (probably Rhizobium) was strongly supported by enzyme electrophoresis; (ii) esterases were more electrophoretically polymorphic than metabolic enzymes, since 35 and 11 electrophoretic types, respectively, were detected within the 57 strains studied; (iii) strains isolated from stem or root nodules were genetically very similar and could not be differentiated; (iv) six Azorhizobium strains isolated from plants growing in saline soils could not be grouped separately from the other strains, which might be attributed to the adaptation of azorhizobia to epiphytic conditions; and (v) a comparative study of esterase patterns of azorhizobia showed that strains isolated in the Philippines probably originated in northern Senegal, but did not reveal a clear separation between strains originating from northern and central Senegal.