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[Determination of mono-, di- and triglycerides using HPLC]
1Chemisch-technologische Hochschule, Institut für Milch- und Fettechnologie, Praha.
Zeitschrift Fur Lebensmittel-Untersuchung Und -Forschung
|June 1, 1993
Summary
This study details a High-Performance Liquid Chromatography (HPLC) method for analyzing glycerides. The method effectively separates monoglycerides, diglycerides, and triglycerides based on molecular mass and ECN1 values.
Area of Science:
- Analytical Chemistry
- Lipid Chemistry
Background:
- Accurate quantification of glycerides (monoglycerides, diglycerides, triglycerides) is crucial in food and lipid analysis.
- Existing methods may require complex sample preparation or lack specificity for different glyceride types.
Purpose of the Study:
- To develop and validate a High-Performance Liquid Chromatography (HPLC) method for the separation and determination of glycerides.
- To investigate the influence of molecular mass and ECN1 values on glyceride separation.
- To estimate response factors for various glycerides.
Main Methods:
- Utilized a normal phase system with Separon SGX-CN (silica gel with phase-bonded cyanoethyl) column.
- Employed a mobile phase consisting of hexane, isopropanol, and formic acid.
- Detected glycerides using HPLC with refractometric detection.
Main Results:
- Successful separation of monoglycerides, diglycerides, and triglycerides based on molecular mass.
- Partial separation of diglycerides and monoglycerides according to ECN1 values was observed.
- Response factors were estimated and found to be dependent on the number of double bonds in the fatty acids.
Conclusions:
- The developed HPLC method provides effective separation of glycerides.
- Molecular mass and ECN1 values are key factors influencing glyceride separation.
- Fatty acid unsaturation impacts response factor values in glyceride analysis.