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Related Experiment Videos

A lactobionate-based extracellular-type solution for donor heart preservation

N Shiiya1, M Paul, C Benvenuti

  • 1Centre de Recherches Chirurgicales, CHU Henri-Mondor, Creteil, France.

The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation
|May 1, 1993
PubMed
Summary

A new lactobionate-based extracellular solution improved heart preservation by maintaining ventricular compliance and adenosine triphosphate (ATP) levels better than University of Wisconsin solution and crystalloid cardioplegia.

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Area of Science:

  • Cardiology
  • Organ Preservation
  • Biochemistry

Background:

  • The University of Wisconsin (UW) solution, an intracellular-type electrolyte solution, is known to improve donor heart preservation.
  • Concerns exist regarding potential deleterious effects of intracellular-type solutions.
  • A novel lactobionate-based extracellular-type solution was developed to address these concerns.

Purpose of the Study:

  • To evaluate a new lactobionate-based extracellular-type solution for donor heart preservation.
  • To compare its efficacy against the University of Wisconsin solution and a crystalloid cardioplegic solution.
  • To assess its impact on ventricular compliance and adenosine triphosphate (ATP) levels.

Main Methods:

  • 45 rabbit hearts were used in the study.

Related Experiment Videos

  • Hearts were arrested using three different solutions: lactobionate-based extracellular-type solution, University of Wisconsin solution, and crystalloid cardioplegic solution.
  • Hearts were immersed in their respective solutions or lactated Ringer's solution for 6 hours at 4°C, followed by reperfusion via a Langendorff circuit.

    • Main Results:

    • Both lactobionate-based solutions demonstrated superior preservation of left ventricular compliance compared to the crystalloid cardioplegic solution.
    • The lactobionate-based extracellular-type solution showed the best preservation of adenosine triphosphate (ATP) levels post-arrest and post-reperfusion.
    • The University of Wisconsin solution exhibited fewer reperfusion arrhythmias.

    Conclusions:

    • Lactobionate plays a crucial role in preserving ventricular compliance during organ preservation.
    • The novel lactobionate-based extracellular-type solution is a promising alternative for enhancing ATP preservation.
    • Further research is needed to investigate factors contributing to reperfusion arrhythmias with different preservation solutions.