Determination of ascorbic acid by isotachophoresis with regard to its potential in neuroblastoma therapy
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S Gebhardt1, K Kraft, H N Lode
1Children's Hospital, University of Tübingen, Germany.
Journal of Chromatography
|May 28, 1993
Abstract:
Analytical capillary isotachophoresis was used to determine ascorbic acid (AA) in different matrices (cell-free system, neuroblastoma cell extracts and urine). The system for purging bone marrow of neuroblastoma cells, including 6-hydroxydopamine (6-OHDA) and AA, was analysed with regard to the interaction of AA with 6-OHDA and its autoxidation product, hydrogen peroxide. Furthermore, analyses concerning the uptake of AA into neuroblastoma cells as well as its excretion in urine after uptake of large amounts were carried out.