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Incomplete coverage of mammalian bone matrix by lining cells
1Department of Otolaryngology, University of California, Davis 95616.
The Annals of Otology, Rhinology, and Laryngology
|July 1, 1993
Summary
Bone lining cells may not form a barrier separating bone fluid from general extracellular fluid. This challenges current theories on osteoclast recruitment and calcium homeostasis regulation.
Area of Science:
- Bone biology
- Cellular physiology
- Calcium homeostasis
Background:
- A functional barrier, potentially formed by bone lining cells, is believed to separate bone extracellular fluid from general extracellular fluid.
- This barrier is theorized to play a role in osteoclast recruitment and calcium homeostasis.
Purpose of the Study:
- To reevaluate the role of bone lining cells in osteoclast recruitment and calcium homeostasis.
- To investigate the structural relationship between bone and extracellular fluid using ultrastructural studies.
Main Methods:
- Ultrastructural studies were conducted on resting bone.
- Quantitative analysis of bone surface contact with extracellular fluid was performed in specific cochlear regions.
Main Results:
- Ultrastructural studies revealed significant portions of resting bone are in direct contact with general extracellular fluid.
- In some cochlear regions, up to 88% of the bone surface directly interfaces with extracellular fluid.
Conclusions:
- The existence of a complete barrier formed by bone lining cells is questionable.
- Current theories on bone lining cells' role in osteoclast recruitment and calcium homeostasis require critical reevaluation based on these anatomic findings.