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Related Experiment Videos

Dihydralazine sulfate analysis using 2-methyl-o-nitropyridine-6-carboxaldehyde

A F Youssef, S A Ibrahim, S R Elshabouri

    Journal of Pharmaceutical Sciences
    |January 1, 1977
    PubMed
    Summary

    A new colorimetric assay accurately quantifies dihydralazine sulfate using a simple reaction. This method is sensitive, selective, and suitable for analyzing dihydralazine sulfate in pharmaceutical formulations.

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    Area of Science:

    • Analytical Chemistry
    • Pharmaceutical Analysis

    Background:

    • Dihydralazine sulfate requires accurate quantification for therapeutic efficacy and safety.
    • Existing analytical methods may lack sensitivity, selectivity, or applicability to complex formulations.

    Purpose of the Study:

    • To develop a sensitive and selective colorimetric assay for dihydralazine sulfate.
    • To validate the assay for quantitative analysis in pharmaceutical products and as a stability-indicating method.

    Main Methods:

    • A colorimetric reaction between dihydralazine sulfate and 2-methyl-3-nitropyridine-6-carboxaldehyde at pH 4.
    • Spectrophotometric quantification of the resulting orange color at 450 nm.
    • Analysis of dihydralazine sulfate in commercial tablet formulations, including stability studies.

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    Main Results:

    • The assay demonstrated high sensitivity with a lower limit of detection of 1 µg/ml.
    • The developed method showed no interference from common excipients or other drugs.
    • The color produced was stable for at least 24 hours.
    • Successful determination of dihydralazine sulfate in various commercial tablets was achieved.

    Conclusions:

    • A novel, sensitive, and selective colorimetric assay for dihydralazine sulfate has been successfully developed.
    • The method is robust, interference-free, and applicable for quality control of pharmaceutical formulations.
    • The assay is suitable for use as a stability-indicating method, ensuring drug integrity over time.