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Tests of sperm function for evaluation of the male: Penetrak and Tru-Trax
C S Niederberger1, D J Lamb, M Glinz
1Scott Department of Urology, Baylor College of Medicine, Houston, Texas 77030.
Objective:
To evaluate the Penetrak (Serono Laboratories, Norwell, MA) and Tru-Trax (Humagen, Charlottesville, VA) bovine cervical mucus (CM) penetration assays and to correlate results to parameters of semen analysis and male factor diagnosis.
Design:
Prospective, nonrandomized, controlled study.
Participants:
Two hundred thirty-six males undergoing evaluation for male factor infertility and 37 fertile semen donors.
Main Outcome Measures:
Human sperm penetration in bovine CM measured by the Penetrak and Tru-Trax assay systems correlated to sperm density, motility, forward progression, and clinical diagnosis.
Results:
Two parameters of semen analysis, motility and density were correlated to the distance traversed by the vanguard sperm in both Penetrak and Tru-Trax assays. Penetrak results were significantly correlated to male factor infertility diagnosis.
Conclusions:
Both assays were reliable and highly reproducible, and the Tru-Trax assay was correlated to its predecessor, Penetrak. Although Penetrak and Tru-Trax were correlated to motility and density, no correlation coefficient was > 0.6, suggesting that these assays measure a facet of sperm function that is independent of semen analysis.