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Related Experiment Videos

NDC1: a nuclear periphery component required for yeast spindle pole body duplication

M Winey1, M A Hoyt, C Chan

  • 1Department of Molecular, Cellular, and Developmental Biology, University of Colorado-Boulder 80309-0347.

The Journal of Cell Biology
|August 1, 1993
PubMed
Summary

The NDC1 gene is essential for spindle pole body (SPB) duplication in Saccharomyces cerevisiae. Mutations disrupt SPB insertion into the nuclear envelope, causing cell cycle defects and asymmetric chromosome segregation.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • The spindle pole body (SPB) in Saccharomyces cerevisiae functions as the centrosome, crucial for mitotic spindle organization.
  • The ndc1-1 mutation is known to cause asymmetric chromosome segregation during mitosis.

Purpose of the Study:

  • To investigate the molecular mechanism underlying the SPB duplication defect in ndc1-1 mutants.
  • To elucidate the function of the NDC1 gene in SPB biogenesis and nuclear envelope integration.

Main Methods:

  • Electron microscopy to examine SPB structure in mutant cells at non-permissive temperatures.
  • Order-of-function experiments to determine the cell cycle requirement for NDC1.
  • Molecular analysis of the NDC1 gene and protein localization studies using immunofluorescence.

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Main Results:

  • Mutant cells exhibit a defect in the insertion of the nascent SPB into the nuclear envelope, despite successful duplication.
  • NDC1 function is required during the G1 phase of the cell cycle.
  • The NDC1 gene encodes a transmembrane protein localized to the nuclear envelope.

Conclusions:

  • The NDC1 protein is essential for mediating the insertion of the duplicated SPB into the nuclear envelope.
  • NDC1 likely plays a critical role in nuclear envelope dynamics during SPB duplication and cell division.