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Ornithine decarboxylase may function as an initiation factor for RNA polymerase I
Science (New York, N.Y.)
|February 4, 1977
Summary
Ornithine decarboxylase, a labile protein, enhances RNA polymerase I activity by increasing initiation rates. This study identifies ornithine decarboxylase as the key modulator of RNA polymerase I, demonstrating its purification via affinity chromatography.
Area of Science:
- Biochemistry
- Molecular Biology
- Enzymology
Background:
- RNA polymerase I activity is regulated by labile proteins with short half-lives.
- Ornithine decarboxylase (ODC) is a labile protein whose activity precedes increased RNA polymerase I activity.
Purpose of the Study:
- To investigate the role of ornithine decarboxylase (ODC) in modulating RNA polymerase I activity.
- To demonstrate a novel purification method for ODC using RNA polymerase I affinity chromatography.
- To provide evidence supporting the hypothesis that ODC is the labile protein regulating RNA polymerase I.
Main Methods:
- RNA polymerase I activity assays.
- Purification of ornithine decarboxylase (ODC) via RNA polymerase I affinity chromatography.
- Analysis of RNA synthesis using radiolabeled adenosine triphosphate incorporation.
Main Results:
- Ornithine decarboxylase (ODC) addition increased the initial rate and linearity of RNA polymerase I assays.
- ODC enhanced the rate of RNA chain initiation.
- A novel method for ODC purification using RNA polymerase I affinity chromatography was established.
Conclusions:
- Ornithine decarboxylase (ODC) directly modulates RNA polymerase I activity.
- ODC is identified as the labile protein responsible for regulating RNA polymerase I.
- RNA polymerase I affinity chromatography is an effective method for ODC purification.