Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Corneal preservation: recent advances

D L Van Horn, R O Schultz

    Survey of Ophthalmology
    |January 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    New corneal preservation methods improve donor tissue availability for penetrating keratoplasty. McCarey-Kaufman medium and cryopreservation offer good clinical results, while organ culture shows future promise.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Chromosome behavior in a cattail millet X Napier grass cross.

    Proceedings of the annual meeting. Hawaiian Academy of Science·2010
    Same author

    Screening for glaucoma with stereo disc photography.

    Journal of glaucoma·2009
    Same author

    Effects of external beam irradiation on the TRAM flap: an experimental model.

    Plastic and reconstructive surgery·2001
    Same author

    Accuracy of glaucoma detection with frequency-doubling perimetry.

    American journal of ophthalmology·2000
    Same author

    Accuracy of scanning laser polarimetry in the diagnosis of glaucoma.

    Archives of ophthalmology (Chicago, Ill. : 1960)·1999
    Same author

    A new technique for the treatment of flexor digitorum profundus tendon avulsion.

    Annals of plastic surgery·1999
    Same journal

    Trends in pediatric uveitis: A systematic review and meta-epidemiological investigation of geographic, economic, and climate factors.

    Survey of ophthalmology·2026
    Same journal

    Efficacy and safety of different intraocular therapies for non-infectious uveitis: A network meta-analysis.

    Survey of ophthalmology·2026
    Same journal

    Macular telangiectasia masqueraders.

    Survey of ophthalmology·2026
    Same journal

    Utilization of anterior segment optical coherence tomography in childhood glaucoma: A systematic review.

    Survey of ophthalmology·2026
    Same journal

    Peripapillary pachychoroid syndrome: Clinical and imaging features, diagnostic differentiation and therapeutic strategies.

    Survey of ophthalmology·2026
    Same journal

    Prognostic factors and postoperative outcomes in pediatric cataract patients: A systematic review and meta-analysis.

    Survey of ophthalmology·2026
    See all related articles

    Area of Science:

    • Ophthalmology
    • Transplantation Biology
    • Tissue Engineering

    Background:

    • Increasing demand for donor corneal tissue necessitates advanced preservation methods for penetrating keratoplasty.
    • Evaluating endothelial viability and clinical outcomes is crucial for assessing corneal preservation techniques.

    Purpose of the Study:

    • To discuss new methods for corneal preservation.
    • To evaluate laboratory techniques for assessing corneal endothelium viability.
    • To review clinical results associated with different preservation methods.

    Main Methods:

    • Corneal excision from donor eyes is required for all discussed preservation methods.
    • Methods evaluated include storage in serum, McCarey-Kaufman (M-K) medium, cryopreservation, and organ culture.

    Related Experiment Videos

  • Laboratory viability assessments and clinical outcome analyses were performed.

    • Main Results:

    • McCarey-Kaufman (M-K) medium storage is simple, inexpensive, and maintains endothelial viability for approximately 4 days.
    • Cryopreservation allows long-term donor tissue storage with good clinical results reported.
    • Organ culture, while experimental, shows potential for maintaining endothelial viability for 3-4 weeks.

    Conclusions:

    • McCarey-Kaufman medium and cryopreservation are effective for corneal preservation in penetrating keratoplasty.
    • Organ culture presents a promising, albeit complex, method for extended corneal tissue viability.
    • Continued research into corneal preservation techniques is vital to meet transplant demands.