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RepFIB: a basic replicon of large plasmids

M D Gibbs1, A J Spiers, P L Bergquist

  • 1Department of Cellular & Molecular Biology, University of Auckland, New Zealand.

Plasmid
|May 1, 1993
PubMed
Summary
This summary is machine-generated.

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The RepFIB replicon is found in various plasmid incompatibility groups, primarily IncF. Sequence analysis reveals high conservation in the RepA protein and flanking DNA repeats, suggesting evolutionary relationships.

Area of Science:

  • Molecular Biology
  • Plasmid Biology
  • Evolutionary Genetics

Background:

  • Plasmids are extrachromosomal DNA elements crucial for bacterial adaptation.
  • Replicons, such as RepFIB, dictate plasmid inheritance and stability.
  • Understanding replicon distribution and evolution is key to plasmid biology.

Purpose of the Study:

  • To determine the distribution of the RepFIB replicon across different plasmid incompatibility groups.
  • To analyze the sequence conservation of the RepFIB minimal replicon region, including the repA gene.
  • To infer the evolutionary relationships among different RepFIB examples.

Main Methods:

  • Southern blot and polymerase chain reaction (PCR) analysis to detect RepFIB presence.
  • DNA sequencing of the minimal replicon region (repA gene and flanking repeats).

Related Experiment Videos

  • Maximum parsimony analysis of sequence data to reconstruct evolutionary relationships.

    • Main Results:

    • RepFIB was detected in 25 out of 55 tested plasmids, spanning multiple incompatibility groups.
    • The majority of RepFIB-carrying plasmids belong to the IncF group, with exceptions in IncI and IncP.
    • Sequence analysis showed high conservation of the RepA protein and flanking DNA repeats across 10 RepFIB examples.

    Conclusions:

    • The RepFIB replicon exhibits a broad host range, not strictly limited to IncF plasmids.
    • Conserved sequences suggest strong selective pressure maintaining the functional integrity of RepFIB.
    • Phylogenetic analysis provides insights into the evolutionary history and diversification of RepFIB replicons.