[Glucocorticoid receptor-like antigen on neuronal plasma membrane of rat brain]

The existence of glucocorticoid receptor-like antigen on the synaptic plasma membrane (SPM) isolated from rat brain was demonstrated by ABC-gold technique. The SPM was first incubated with McAb 250 (a monoclonal antibody to the glucocorticoid receptor) and then biotinylated horse-anti-mouse IgG and streptavidin-gold (SAG) was added in proper order to the reacting system. The gold particles could be visualized on the external surface of neuronal processes under electron microscope. This is the first morphological demonstration of the existence of glucocorticoid receptor-like antigen on neuronal membranes.