Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microbiosensors for acetylcholine and glucose

I Karube1, K Yokoyama, E Tamiya

  • 1Research Center for Advanced Science and Technology, University of Tokyo, Japan.

Biosensors & Bioelectronics
|January 1, 1993
PubMed
Summary

New microbiosensors using carbon and platinum fibers offer sensitive detection of hydrogen peroxide and glucose. These advanced sensors demonstrate reliable performance in complex samples and provide wider linear ranges for accurate measurements.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An optical pickup enzyme-linked immunosorbent assay (ELISA) with a microfluidic disk.

RSC advances·2022
Same author

Centrifugal microfluidic platform for single-cell level cardiomyocyte-based drug profiling and screening.

Lab on a chip·2015
Same author

Recent trends of biosensors in Japan.

Applied biochemistry and biotechnology·2013
Same author

Micromachined severinghaus-type carbon dioxide electrode.

Analytical chemistry·2011
Same author

Optical detection of chloramphenicol using molecularly imprinted polymers.

Analytical chemistry·2011
Same author

Molecularly imprinted polymers--tyrosinase mimics.

Ukrains'kyi biokhimichnyi zhurnal (1999 )·2009

Area of Science:

  • Electrochemistry
  • Biosensors
  • Analytical Chemistry

Background:

  • Development of sensitive and selective microbiosensors is crucial for various analytical applications.
  • Existing methods for detecting analytes like hydrogen peroxide and glucose face challenges in sensitivity, selectivity, and sample matrix interference.

Purpose of the Study:

  • To develop and characterize microbiosensors based on carbon and platinum fibers for the determination of hydrogen peroxide and glucose.
  • To evaluate the performance of these microbiosensors in terms of sensitivity, selectivity, reproducibility, and linear range.
  • To investigate the influence of immobilization techniques and mediators on sensor performance.

Main Methods:

  • Fabrication of microelectrodes using 7-micrometer carbon fibers and 2-micrometer cylindrical platinum fibers.

Related Experiment Videos

  • Immobilization of enzymes (acetylcholine esterase, choline oxidase, glucose oxidase, glucose dehydrogenase) using poly(vinyl alcohol)-quarternized stilbazole (PVA-SbQ).
  • Electrochemical characterization using cyclic voltammetry, chronoamperometry, and specific potential pulse sequences for analyte detection.

    • Main Results:

    • Carbon fiber microelectrodes achieved a 0.1 microM determination limit for hydrogen peroxide, with successful separation from ascorbic acid and stable detection in albumin.
    • An acetylcholine microsensor exhibited a linear range of 0.1-1.0 mM with a correlation coefficient of 0.9842.
    • Glucose microsensors based on glucose oxidase (GOD) and glucose dehydrogenase (GDH) showed wider linear ranges and enhanced responses, particularly the GDH sensor with diaphorase, unaffected by dissolved oxygen.

    Conclusions:

    • Microbiosensors fabricated with carbon and platinum fibers offer high sensitivity and selectivity for target analytes.
    • The immobilization strategy and choice of mediators significantly impact sensor performance, enabling wider linear ranges and improved detection limits.
    • These microbiosensors show promise for reliable and sensitive electrochemical analysis in complex biological samples.