Related Experiment Video
Updated: Jul 24, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Phylogenetic grouping and identification of Rhizobium isolates on the basis of random amplified polymorphic DNA
J J Dooley1, S P Harrison, L R Mytton
1Royal Agricultural College, Cirencester, Gloucestershire, U.K.
Abstract:
Through the use of a single, random 15mer as a primer, between 1 and 12 DNA amplification products were obtained per strain from a selection of 84 Rhizobium and Bradyrhizobium isolates. A principal-coordinate analysis was used to analyse the resulting amplified DNA profiles and it was possible to assign isolates to specific groupings. Within the species Rhizobium leguminosarum, the biovar phaseoli formed a distinct group from the other biovars of the species, viciae and trifolii, which grouped together. Isolates of Rhizobium meliloti and Bradyrhizobium species formed their own clear, specific groups. Although it was possible to identify individual isolates on the basis of differences in their amplified DNA profiles, there was evidence that some amplified segments were conserved among individuals at the biovar and species levels.
Related Concept Videos
Methods of Classification and Identification
Modern Molecular Taxonomy
Applications of Molecular Taxonomy
Diversity of Protists III
Microbial Phylogeny
Rapid Identification of Pathogens

