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NGFI-A gene expression is necessary for T lymphocyte proliferation
A Perez-Castillo1, C Pipaón, I García
1Insituto Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
The Journal of Biological Chemistry
|September 15, 1993
Summary
The immediate early gene NGFI-A is crucial for lymphocyte proliferation. Its expression, triggered by IL-2 or ConA, is essential for T cell growth and mitogenic stimulation.
Area of Science:
- Molecular Biology
- Immunology
- Cell Biology
Background:
- NGFI-A is an immediate early gene encoding a zinc finger protein.
- It is rapidly activated following mitogenic stimulation in lymphocytes.
Purpose of the Study:
- To investigate the role of NGFI-A gene expression in lymphocyte proliferation.
- To understand the signaling pathways regulating NGFI-A induction.
Main Methods:
- Stimulation of lymphoblasts and T lymphocytes with Interleukin-2 (IL-2) and concanavalin A (ConA).
- Analysis of NGFI-A gene expression using signaling pathway activators (Ca2+, protein kinase C, 8-Bromo-cAMP).
- Inhibition of NGFI-A expression with antisense oligonucleotides.
Main Results:
- NGFI-A expression was rapidly induced by IL-2 in G1 lymphoblasts and during G0/G1 transition with ConA.
- Full NGFI-A induction required combined Ca2+ and protein kinase C activation in quiescent T lymphocytes.
- NGFI-A antisense oligonucleotide blocked ConA- and IL-2-induced lymphocyte proliferation.
Conclusions:
- NGFI-A plays a critical regulatory role in lymphocyte growth control.
- NGFI-A expression is essential for T cell proliferation following mitogenic stimulation.