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Human amnion metabolism. I. In vitro maintenance
American Journal of Obstetrics and Gynecology
|March 1, 1977
Summary
Human amnion tissue is metabolically active, storing glycogen and ATP. It remains viable in a simulated amniotic fluid medium for up to 120 minutes, demonstrating sustained biochemical function.
Area of Science:
- Biochemistry
- Human Physiology
- Cell Biology
Background:
- The human amnion is a fetal membrane that surrounds the amniotic fluid.
- Understanding the metabolic activity of the amnion is crucial for studying fetal development and placental function.
Purpose of the Study:
- To investigate the metabolic activity and viability of human term amnion tissue.
- To determine the energy stores and glucose utilization rates of amnion.
Main Methods:
- Incubation of human term amnion tissue in a defined medium simulating amniotic fluid at 37°C for 120 minutes.
- Measurement of glycogen and adenosine triphosphate (ATP) concentrations.
- Quantification of glucose utilization rates.
Main Results:
- Amnion tissue demonstrated preserved histologic structure and biochemical functions during incubation.
- Glycogen concentrations remained stable, while ATP levels were maintained at 85-100% of control values.
- Linear glucose utilization was observed at a rate of 70.0 +/- 3.0 nmoles/min/g tissue.
Conclusions:
- Human term amnion is a metabolically active tissue.
- The amnion can be maintained in a viable state in a suitable in vitro medium.
- These findings support the potential for further research into amnion's physiological roles.